Firefly Technology Sheds New Light

The light-producing enzyme in the firefly is the key to rapid pathogen detection // Source: cri.cn

submitted by Luis Kun

Homeland Security News Wire - March 22, 2012

A new device, employing the same chemical which lights up fireflies, can easily detect food contamination; the researchers who developed the system hope it will soon be used to test for other diseases, including HIV-AIDS.

Food contamination can now be detected easily by a new device based on the chemical which lights up fireflies.

The Bioluminescent Assay in Real-Time (BART), jointly invented by Professor Jim Murray of the Cardiff University’s School of Biosciences and Dr. Laurence Tisi of Lumora, allows users to test rapidly and simply for food poisoning bacteria. Professor Murray and his partners at technology company Lumora Ltd. hope to develop the system to test for other diseases, including HIV-AIDS.

A Cardiff University release reports that the BART system detects specific DNA sequences by producing a light signal, using a version of the enzyme luciferase, which also produces light in fireflies. The breakthrough allows for quick and easy molecular testing which previously required complex laboratory hardware.

Professor Murray and Dr. Tisi have just been selected as finalists for the Innovator of the Year award run by the Biotechnology and Biological Sciences Research Council.

The first BART system has been created for food safety testing. Samples are placed inside a remarkably simple device, which can then test for the DNA of common food pathogens. If present, the bacteria trigger the luciferase to produce light. The device can produce results in ten minutes to an hour, depending on the number of organisms being tested for.

Professor Murray said: “The food industry has been looking for dependable, fast and convenient microbiological testing for a long time. Our system will allow workers to test a wide variety of foods in a simple system which uses the most sensitive molecular technology. Portable versions of the device mean that it’s now even possible to test farm animals in the food chain.

“We now want to apply BART technology to a range of other diseases. The most obvious and most pressing need is HIV-AIDS. Patients need to be continuously tested for their viral loads so that their treatment dosage can be adjusted. At the moment, this requires laboratory facilities which are in short supply in Africa. We hope to create a device which can be used easily in those countries.”

Lumora is a spin-out company based on Professor Murray’s research which now employs thirteen people in Cambridgeshire.

The results of the BBSRC Innovator of the Year Award will be announced on Wednesday, 28 March.

http://www.homelandsecuritynewswire.com/dr20120321-firefly-technology-sheds-new-light